A pull-down procedure for the identification of unknown GEFs for small GTPases.

ABSTRACT: Members of the family of small GTPases regulate a variety of important cellular functions. In order to accomplish this, tight temporal and spatial regulation is absolutely necessary. The two most important factors for this regulation are GTPase activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs), the latter being responsible for the activation of the GTPase downstream pathways at the correct location and time. Although a large number of exchange factors have been identified, it is likely that a similarly large number remains unidentified. We have therefore developed a procedure to specifically enrich GEF proteins from biological samples making use of the high affinity binding of GEFs to nucleotide-free GTPases. In order to verify the results of these pull-down experim

SUBMITTER: Koch D 

PROVIDER: S-EPMC4905258 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature