A biomimetic synthetic feeder layer supports the proliferation and self-renewal of mouse embryonic stem cells.

ABSTRACT:

Unlabelled

Successful realization of the enormous potential of pluripotent stem cells in regenerative medicine demands the development of well-defined culture conditions. Maintenance of embryonic stem cells (ESCs) typically requires co-culture with feeder layer cells, generally mouse embryonic fibroblasts (MEFs). Concerns about xenogeneic pathogen contamination and immune reaction to feeder cells underlie the need for ensuring the safety and efficacy of future stem cell-based products through the development of a controlled culture environment. To gain insight into the effectiveness of MEF layers, here we have developed a biomimetic synthetic feeder layer (BSFL) that is acellular and replicates the stiffness and topography of MEFs. The mechanical properties of MEFs were measured us

SUBMITTER: Lopez-Fagundo C 

PROVIDER: S-EPMC4905775 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature