FNA smears as a potential source of DNA for targeted next-generation sequencing of lung adenocarcinomas.

ABSTRACT:

Background

Diff-Quik-stained fine-needle aspiration (FNA) smears and touch preparations from biopsies represent alternative specimens for molecular testing when cell block or biopsy material is insufficient. This study describes the use of these samples for targeted next-generation sequencing (NGS) of primary and metastatic lung adenocarcinoma and reports the DNA quality and success rates of FNA smears versus other specimens from 1 year of clinical use.

Methods

A validation set of 10 slides from 9 patients with prior clinical epidermal growth factor receptor (EGFR) Sanger sequencing and KRAS pyrosequencing (5 KRAS-positive/EGFR-negative and 4 KRAS-negative/EGFR-negative) underwent DNA extraction, quality assessment, and targeted NGS. Subsequently, lung adenocarcinoma specime

SUBMITTER: Treece AL 

PROVIDER: S-EPMC4905788 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature