Super-resolution imaging reveals distinct chromatin folding for different epigenetic states.

ABSTRACT: Metazoan genomes are spatially organized at multiple scales, from packaging of DNA around individual nucleosomes to segregation of whole chromosomes into distinct territories. At the intermediate scale of kilobases to megabases, which encompasses the sizes of genes, gene clusters and regulatory domains, the three-dimensional (3D) organization of DNA is implicated in multiple gene regulatory mechanisms, but understanding this organization remains a challenge. At this scale, the genome is partitioned into domains of different epigenetic states that are essential for regulating gene expression. Here we investigate the 3D organization of chromatin in different epigenetic states using super-resolution imaging. We classified genomic domains in Drosophila cells into transcriptionally active, inac

SUBMITTER: Boettiger AN 

PROVIDER: S-EPMC4905822 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature