Direct polymerase chain reaction from blood and tissue samples for rapid diagnosis of bovine leukemia virus infection.

ABSTRACT: Bovine leukemia virus (BLV) infection induces bovine leukemia in cattle and causes significant financial harm to farmers and farm management. There is no effective therapy or vaccine; thus, the diagnosis and elimination of BLV-infected cattle are the most effective method to eradicate the infection. Clinical veterinarians need a simpler and more rapid method of diagnosing infection, because both nested polymerase chain reaction (PCR) and real-time PCR are labor intensive, time-consuming, and require specialized molecular biology techniques and expensive equipment. In this study, we describe a novel PCR method for amplifying the BLV provirus from whole blood, thus eliminating the need for DNA extraction. Although the sensitivity of PCR directly from whole blood (PCR-DB) samples as measured

SUBMITTER: Nishimori A 

PROVIDER: S-EPMC4905833 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature