An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1.

ABSTRACT: Measurement of RNA can be used to study and monitor a range of infectious and non-communicable diseases, with profiling of multiple gene expression mRNA transcripts being increasingly applied to cancer stratification and prognosis. An international comparison study (Consultative Committee for Amount of Substance (CCQM)-P103.1) was performed in order to evaluate the comparability of measurements of RNA copy number ratio for multiple gene targets between two samples. Six exogenous synthetic targets comprising of External RNA Control Consortium (ERCC) standards were measured alongside transcripts for three endogenous gene targets present in the background of human cell line RNA. The study was carried out under the auspices of the Nucleic Acids (formerly Bioanalysis) Working Group of the CCQM.

SUBMITTER: Devonshire AS 

PROVIDER: S-EPMC4906133 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature