Reorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy.

ABSTRACT: Myelin is a multilayered membrane that ensheathes axonal fibers in the vertebrate nervous system, allowing fast propagation of nerve action potentials. It contains densely packed lipids, lacks an actin-based cytocortex, and requires myelin basic protein (MBP) as its major structural component. This protein is the basic constituent of the proteinaceous meshwork that is localized between adjacent cytoplasmic membranes of the myelin sheath. Yet, it is not clear how MBP influences the organization and dynamics of the lipid constituents of myelin. Here, we used optical stimulated emission depletion super-resolution microscopy in combination with fluorescence correlation spectroscopy to assess the characteristics of diffusion of different fluorescent lipid analogs in myelin membrane sheets of cu

SUBMITTER: Steshenko O 

PROVIDER: S-EPMC4906378 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature