A pH-Mediated Topological Switch within the N-Terminal Domain of Human Caveolin-3.

ABSTRACT: Caveolins mediate the formation of caveolae, which are small omega-shaped membrane invaginations involved in a variety of cellular processes. There are three caveolin isoforms, the third of which (Cav3) is expressed in smooth and skeletal muscles. Mutations in Cav3 cause a variety of human muscular diseases. In this work, we characterize the secondary structure, dynamics, and topology of the monomeric form of the full-length lipidated protein. Cav3 consists of a series of membrane-embedded or surface-associated helical elements connected by extramembrane connecting loops or disordered domains. Our results also reveal that the N-terminal domain undergoes a large scale pH-mediated topological rearrangement between soluble and membrane-anchored forms. Considering that roughly one-third of pat

SUBMITTER: Kim JH 

PROVIDER: S-EPMC4906379 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature