Evaluation of fliC-d based direct blood PCR assays for typhoid diagnosis.

ABSTRACT:

Background

Typhoid cases need to be diagnosed accurately for early antibiotic therapy and reducing mortality. Identification of Salmonella Typhi (S. Typhi) in blood culture is conclusive, but has poor sensitivity. Detection of S. Typhi by PCR from blood sample has shown promise. Real-time quantitative PCR (Q-PCR) has been widely used in diagnostics for its rapidity and reliability. In the present study, the performance of molecular methods like conventional PCR (C-PCR), nested PCR (N-PCR) and Q-PCR were investigated and compared by targeting S. Typhi specific flagellar fliC-d gene directly in blood samples for typhoid diagnosis.

Results

Analytical sensitivities and specificities of the PCR assays were determined under laboratory condition followed by diagnostic performances

SUBMITTER: Das S 

PROVIDER: S-EPMC4906692 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature