Genetic Diversity of Crimean Congo Hemorrhagic Fever Virus Strains from Iran.

ABSTRACT:

Background

Crimean Congo hemorrhagic fever virus (CCHFV) is a member of the Bunyaviridae family and Nairovirus genus. It has a negative-sense, single stranded RNA genome approximately 19.2 kb, containing the Small, Medium, and Large segments. CCHFVs are relatively divergent in their genome sequence and grouped in seven distinct clades based on S-segment sequence analysis and six clades based on M-segment sequences. Our aim was to obtain new insights into the molecular epidemiology of CCHFV in Iran.

Methods

We analyzed partial and complete nucleotide sequences of the S and M segments derived from 50 Iranian patients. The extracted RNA was amplified using one-step RT-PCR and then sequenced. The sequences were analyzed using Mega5 software.

Results

Phylogenetic analysis

SUBMITTER: Chinikar S 

PROVIDER: S-EPMC4906752 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature