Highly Branched Pentasaccharide-Bearing Amphiphiles for Membrane Protein Studies.

ABSTRACT: Detergents are essential tools for membrane protein manipulation. Micelles formed by detergent molecules have the ability to encapsulate the hydrophobic domains of membrane proteins. The resulting protein-detergent complexes (PDCs) are compatible with the polar environments of aqueous media, making structural and functional analysis feasible. Although a number of novel agents have been developed to overcome the limitations of conventional detergents, most have traditional head groups such as glucoside or maltoside. In this study, we introduce a class of amphiphiles, the PSA/Es with a novel highly branched pentasaccharide hydrophilic group. The PSA/Es conferred markedly increased stability to a diverse range of membrane proteins compared to conventional detergents, indicating a positive rol

SUBMITTER: Ehsan M 

PROVIDER: S-EPMC4906958 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature