The Regulation of rRNA Gene Transcription during Directed Differentiation of Human Embryonic Stem Cells.

ABSTRACT: It has become increasingly clear that proper cellular control of pluripotency and differentiation is related to the regulation of rRNA synthesis. To further our understanding of the role that the regulation of rRNA synthesis has in pluripotency we monitored rRNA synthesis during the directed differentiation of human embryonic stem cells (hESCs). We discovered that the rRNA synthesis rate is reduced ~50% within 6 hours of ACTIVIN A treatment. This precedes reductions in expression of specific stem cell markers and increases in expression of specific germ layer markers. The reduction in rRNA synthesis is concomitant with dissociation of the Pol I transcription factor, UBTF, from the rRNA gene promoter and precedes any increase to heterochromatin throughout the rRNA gene. To directly investig

SUBMITTER: Woolnough JL 

PROVIDER: S-EPMC4907514 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature