KymographClear and KymographDirect: two tools for the automated quantitative analysis of molecular and cellular dynamics using kymographs.

ABSTRACT: Dynamic processes are ubiquitous and essential in living cells. To properly understand these processes, it is imperative to measure them in a time-dependent way and analyze the resulting data quantitatively, preferably with automated tools. Kymographs are single images that represent the motion of dynamic processes and are widely used in live-cell imaging. Although they contain the full range of dynamics, it is not straightforward to extract this quantitative information in a reliable way. Here we present two complementary, publicly available software tools, KymographClear and KymographDirect, that have the power to reveal detailed insight in dynamic processes. KymographClear is a macro toolset for ImageJ to generate kymographs that provides automatic color coding of the different directio

SUBMITTER: Mangeol P 

PROVIDER: S-EPMC4907728 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature