Signal Propagation between Neuronal Populations Controlled by Micropatterning.

ABSTRACT: The central nervous system consists of an unfathomable number of functional networks enabling highly sophisticated information processing. Guided neuronal growth with a well-defined connectivity and accompanying polarity is essential for the formation of these networks. To investigate how two-dimensional protein patterns influence neuronal outgrowth with respect to connectivity and functional polarity between adjacent populations of neurons, a microstructured model system was established. Exclusive cell growth on patterned substrates was achieved by transferring a mixture of poly-l-lysine and laminin to a cell-repellent glass surface by microcontact printing. Triangular structures with different opening angle, height, and width were chosen as a pattern to achieve network formation with def

SUBMITTER: Albers J 

PROVIDER: S-EPMC4908115 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature