Digital Gene Expression Analysis of Populus simonii × P. nigra Pollen Germination and Tube Growth.

ABSTRACT: Pollen tubes are an ideal model for the study of cell growth and morphogenesis because of their extreme elongation without cell division; however, the genetic basis of pollen germination and tube growth remains largely unknown. Using the Illumina/Solexa digital gene expression system, we identified 13,017 genes (representing 28.3% of the unigenes on the reference genes) at three stages, including mature pollen, hydrated pollen, and pollen tubes of Populus simonii × P. nigra. Comprehensive analysis of P. simonii × P. nigra pollen revealed dynamic changes in the transcriptome during pollen germination and pollen tube growth (PTG). Gene ontology analysis of differentially expressed genes showed that genes involved in functional categories such as catalytic activity, binding, transporter activ

SUBMITTER: Zhao LJ 

PROVIDER: S-EPMC4908133 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature