A proposed integrated approach for the preclinical evaluation of phage therapy in Pseudomonas infections.

ABSTRACT: Bacteriophage therapy is currently resurging as a potential complement/alternative to antibiotic treatment. However, preclinical evaluation lacks streamlined approaches. We here focus on preclinical approaches which have been implemented to assess bacteriophage efficacy against Pseudomonas biofilms and infections. Laser interferometry and profilometry were applied to measure biofilm matrix permeability and surface geometry changes, respectively. These biophysical approaches were combined with an advanced Airway Surface Liquid infection model, which mimics in vitro the normal and CF lung environments, and an in vivo Galleria larvae model. These assays have been implemented to analyze KTN4 (279,593 bp dsDNA genome), a type-IV pili dependent, giant phage resembling phiKZ. Upon contact, KTN4 i

SUBMITTER: Danis-Wlodarczyk K 

PROVIDER: S-EPMC4908380 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature