A rapid and practical technique for real-time monitoring of biomolecular interactions using mechanical responses of macromolecules.

ABSTRACT: Monitoring biological reactions using the mechanical response of macromolecules is an alternative approach to immunoassays for providing real-time information about the underlying molecular mechanisms. Although force spectroscopy techniques, e.g. AFM and optical tweezers, perform precise molecular measurements at the single molecule level, sophisticated operation prevent their intensive use for systematic biosensing. Exploiting the biomechanical assay concept, we used micro-electro mechanical systems (MEMS) to develop a rapid platform for monitoring bio/chemical interactions of bio macromolecules, e.g. DNA, using their mechanical properties. The MEMS device provided real-time monitoring of reaction dynamics without any surface or molecular modifications. A microfluidic device with a side o

SUBMITTER: Tarhan MC 

PROVIDER: S-EPMC4910067 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature