GRAbB: Selective Assembly of Genomic Regions, a New Niche for Genomic Research.

ABSTRACT: GRAbB (Genomic Region Assembly by Baiting) is a new program that is dedicated to assemble specific genomic regions from NGS data. This approach is especially useful when dealing with multi copy regions, such as mitochondrial genome and the rDNA repeat region, parts of the genome that are often neglected or poorly assembled, although they contain interesting information from phylogenetic or epidemiologic perspectives, but also single copy regions can be assembled. The program is capable of targeting multiple regions within a single run. Furthermore, GRAbB can be used to extract specific loci from NGS data, based on homology, like sequences that are used for barcoding. To make the assembly specific, a known part of the region, such as the sequence of a PCR amplicon or a homologous sequence f

SUBMITTER: Brankovics B 

PROVIDER: S-EPMC4911045 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature