The biochemical origins of the surface-enhanced Raman spectra of bacteria: a metabolomics profiling by SERS.

ABSTRACT: The dominant molecular species contributing to the surface-enhanced Raman spectroscopy (SERS) spectra of bacteria excited at 785 nm are the metabolites of purine degradation: adenine, hypoxanthine, xanthine, guanine, uric acid, and adenosine monophosphate. These molecules result from the starvation response of the bacterial cells in pure water washes following enrichment from nutrient-rich environments. Vibrational shifts due to isotopic labeling, bacterial SERS spectral fitting, SERS and mass spectrometry analysis of bacterial supernatant, SERS spectra of defined bacterial mutants, and the enzymatic substrate dependence of SERS spectra are used to identify these molecular components. The absence or presence of different degradation/salvage enzymes in the known purine metabolism pathways o

SUBMITTER: Premasiri WR 

PROVIDER: S-EPMC4911336 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature