From DNA to FBA: How to Build Your Own Genome-Scale Metabolic Model.

ABSTRACT: Microbiological studies are increasingly relying on in silico methods to perform exploration and rapid analysis of genomic data, and functional genomics studies are supplemented by the new perspectives that genome-scale metabolic models offer. A mathematical model consisting of a microbe's entire metabolic map can be rapidly determined from whole-genome sequencing and annotating the genomic material encoded in its DNA. Flux-balance analysis (FBA), a linear programming technique that uses metabolic models to predict the phenotypic responses imposed by environmental elements and factors, is the leading method to simulate and manipulate cellular growth in silico. However, the process of creating an accurate model to use in FBA consists of a series of steps involving a multitude of connections

SUBMITTER: Cuevas DA 

PROVIDER: S-EPMC4911401 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature