Quantitative lineage tracing strategies to resolve multipotency in tissue-specific stem cells.

ABSTRACT: Lineage tracing has become the method of choice to study the fate and dynamics of stem cells (SCs) during development, homeostasis, and regeneration. However, transgenic and knock-in Cre drivers used to perform lineage tracing experiments are often dynamically, temporally, and heterogeneously expressed, leading to the initial labeling of different cell types and thereby complicating their interpretation. Here, we developed two methods: the first one based on statistical analysis of multicolor lineage tracing, allowing the definition of multipotency potential to be achieved with high confidence, and the second one based on lineage tracing at saturation to assess the fate of all SCs within a given lineage and the "flux" of cells between different lineages. Our analysis clearly shows that, wh

SUBMITTER: Wuidart A 

PROVIDER: S-EPMC4911926 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature