Mapping of Brain Activity by Automated Volume Analysis of Immediate Early Genes.

ABSTRACT: Understanding how neural information is processed in physiological and pathological states would benefit from precise detection, localization, and quantification of the activity of all neurons across the entire brain, which has not, to date, been achieved in the mammalian brain. We introduce a pipeline for high-speed acquisition of brain activity at cellular resolution through profiling immediate early gene expression using immunostaining and light-sheet fluorescence imaging, followed by automated mapping and analysis of activity by an open-source software program we term ClearMap. We validate the pipeline first by analysis of brain regions activated in response to haloperidol. Next, we report new cortical regions downstream of whisker-evoked sensory processing during active exploration. L

SUBMITTER: Renier N 

PROVIDER: S-EPMC4912438 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature