Nucleus downscaling in mouse embryos is regulated by cooperative developmental and geometric programs.

ABSTRACT: Maintaining appropriate nucleus size is important for cell health, but the mechanisms by which this is achieved are poorly understood. Controlling nucleus size is a particular challenge in early development, where the nucleus must downscale in size with progressive reductive cell divisions. Here we use live and fixed imaging, micromanipulation approaches, and small molecule analyses during preimplantation mouse development to probe the mechanisms by which nucleus size is determined. We find a close correlation between cell and nuclear size at any given developmental stage, and show that experimental cytoplasmic reduction can alter nuclear size, together indicating that cell size helps dictate nuclear proportions. Additionally, however, by creating embryos with over-sized blastomeres we pre

SUBMITTER: Tsichlaki E 

PROVIDER: S-EPMC4913252 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature