Application of targeted enrichment to next-generation sequencing of retroviruses integrated into the host human genome.

ABSTRACT: The recent development and advancement of next-generation sequencing (NGS) technologies have enabled the characterization of the human genome at extremely high resolution. In the retrovirology field, NGS technologies have been applied to integration-site analysis and deep sequencing of viral genomes in combination with PCR amplification using virus-specific primers. However, virus-specific primers are not available for some epigenetic analyses, like chromatin immunoprecipitation sequencing (ChIP-seq) assays. Viral sequences are poorly detected without specific PCR amplification because proviral DNA is very scarce compared to human genomic DNA. Here, we have developed and evaluated the use of biotinylated DNA probes for the capture of viral genetic fragments from a library prepared for NGS.

SUBMITTER: Miyazato P 

PROVIDER: S-EPMC4913254 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature