Metatranscriptomics reveals the molecular mechanism of large granule formation in granular anammox reactor.

ABSTRACT: Granules enriched with anammox bacteria are essential in enhancing the treatment of ammonia-rich wastewater, but little is known about how anammox bacteria grow and multiply inside granules. Here, we combined metatranscriptomics, quantitative PCR and 16S rRNA gene sequencing to study the changes in community composition, metabolic gene content and gene expression in a granular anammox reactor with the objective of understanding the molecular mechanism of anammox growth and multiplication that led to formation of large granules. Size distribution analysis revealed the spatial distribution of granules in which large granules having higher abundance of anammox bacteria (genus Brocadia) dominated the bottom biomass. Metatranscriptomics analysis detected all the essential transcripts for anammo

SUBMITTER: Bagchi S 

PROVIDER: S-EPMC4913261 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature