Ultrasensitive proteomic quantitation of cellular signaling by digitized nanoparticle-protein counting.

ABSTRACT: Many important signaling and regulatory proteins are expressed at low abundance and are difficult to measure in single cells. We report a molecular imaging approach to quantitate protein levels by digitized, discrete counting of nanoparticle-tagged proteins. Digitized protein counting provides ultrasensitive molecular detection of proteins in single cells that surpasses conventional methods of quantitating total diffuse fluorescence, and offers a substantial improvement in protein quantitation. We implement this digitized proteomic approach in an integrated imaging platform, the single cell-quantum dot platform (SC-QDP), to execute sensitive single cell phosphoquantitation in response to multiple drug treatment conditions and using limited primary patient material. The SC-QDP: 1) identifie

SUBMITTER: Jacob T 

PROVIDER: S-EPMC4913309 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature