Connexin 50 Regulates Surface Ball-and-Socket Structures and Fiber Cell Organization.

ABSTRACT:

Purpose

The roles of gap junction protein connexin 50 (Cx50) encoded by Gja8, during lens development are not fully understood. Connexin 50 knockout (KO) lenses have decreased proliferation of epithelial cells and altered fiber cell denucleation. We further investigated the mechanism for cellular defects in Cx50 KO (Gja8-/-) lenses.

Methods

Fiber cell morphology and subcellular distribution of various lens membrane/cytoskeleton proteins from wild-type and Cx50 KO mice were visualized by immunofluorescent staining and confocal microscopy.

Results

We observed multiple morphological defects in the cortical fibers of Cx50 KO lenses, including abnormal fiber cell packing geometry, decreased F-actin enrichment at tricellular vertices, and disrupted ball-and-socket (BS) stru

SUBMITTER: Wang E 

PROVIDER: S-EPMC4913802 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature