Mass spectrometry-based absolute quantification reveals rhythmic variation of mouse circadian clock proteins.

ABSTRACT: Absolute values of protein expression levels in cells are crucial information for understanding cellular biological systems. Precise quantification of proteins can be achieved by liquid chromatography (LC)-mass spectrometry (MS) analysis of enzymatic digests of proteins in the presence of isotope-labeled internal standards. Thus, development of a simple and easy way for the preparation of internal standards is advantageous for the analyses of multiple target proteins, which will allow systems-level studies. Here we describe a method, termed MS-based Quantification By isotope-labeled Cell-free products (MS-QBiC), which provides the simple and high-throughput preparation of internal standards by using a reconstituted cell-free protein synthesis system, and thereby facilitates both multiplexe

SUBMITTER: Narumi R 

PROVIDER: S-EPMC4914154 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature