Genotyping of the Helicobacter pylori cagA Gene Isolated From Gastric Biopsies in Shiraz, Southern Iran: A PCR-RFLP and Sequence Analysis Approach.

ABSTRACT:

Background

Cytotoxin-associated gene A (cagA) is an important virulence factor in the pathogenesis of Helicobacter pylori.

Objectives

The aim of this study was to genotype the H. pylori cagA gene isolated from antral biopsies of patients with stomach symptoms, using a PCR-restriction fragment length polymorphism (PCR-RFLP) analysis.

Patients and methods

A total of 161 gastric biopsies were collected from patients with stomach symptoms. After isolation of H. pylori from the biopsy culture, the cagA gene was assessed using PCR. The PCR products were then digested by the HinfI restriction endonuclease enzyme. A sample of each genotype was also subjected to direct sequencing for further analysis.

Results

From 161 antral biopsies, 61 (37.9%) were positive for H. pyl

SUBMITTER: Moaddeb A 

PROVIDER: S-EPMC4914860 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature