Subangstrom single-molecule measurements of motor proteins using a nanopore.

ABSTRACT: Techniques for measuring the motion of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of ∼300 pm. We use ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel308 on DNA with up to ∼40 pm sensitivity. This approach should be applicable to any protein that translocates on DNA or RNA, including helicases, polymerases, recombinases and DNA repair enzymes.

SUBMITTER: Derrington IM 

PROVIDER: S-EPMC4915380 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature