Protection of the Transplant Kidney from Preservation Injury by Inhibition of Matrix Metalloproteinases.

ABSTRACT:

Background

Matrix metalloproteinases (MMPs), particularly MMP-2 and MMP-9, play an important role in ischemic injury to the heart, yet it is not known if these MMPs are involved in the injury that occurs to the transplant kidney. We therefore studied the pharmacologic protection of transplant kidneys during machine cold perfusion.

Methods

Human kidney perfusates were analyzed for the presence of injury markers such as cytochrome c oxidase, lactate dehydrogenase, and neutrophil-gelatinase associated lipocalin (NGAL), and MMP-2 and MMP-9 were measured. The effects of MMP inhibitors MMP-2 siRNA and doxycycline were studied in an animal model of donation after circulatory determination of death (DCDD).

Results

Markers of injury were present in all analyzed perfusates, wit

SUBMITTER: Moser MA 

PROVIDER: S-EPMC4915675 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature