KIF5B and Nup358 Cooperatively Mediate the Nuclear Import of HIV-1 during Infection.

ABSTRACT: Following envelope mediated fusion, the HIV-1 core is released into the cytoplasm of the target cell and undergoes a series of trafficking and replicative steps that result in the nuclear import of the viral genome, which ultimately leads to the integration of the proviral DNA into the host cell genome. Previous studies have found that disruption of microtubules, or depletion of dynein or kinesin motors, perturb the normal uncoating and trafficking of the viral genome. Here, we show that the Kinesin-1 motor, KIF5B, induces a relocalization of the nuclear pore component Nup358 into the cytoplasm during HIV-1 infection. This relocalization of NUP358 is dependent on HIV-1 capsid, and NUP358 directly associates with viral cores following cytoplasmic translocation. This interaction between NUP3

SUBMITTER: Dharan A 

PROVIDER: S-EPMC4915687 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature