Role of tumor necrosis factor-alpha in zebrafish retinal neurogenesis and myelination.

ABSTRACT:

Aim

To investigate the role of tumor necrosis factor-alpha (TNF-α) in zebrafish retinal development and myelination.

Methods

Morpholino oligonucleotides (MO), which are complementary to the translation start site of the wild-type embryonic zebrafish TNF-α mRNA sequence, were synthesized and injected into one- to four-cell embryos. The translation blocking specificity was verified by Western blotting using an anti-TNF-α antibody, whole-mount in situ hybridization using a hepatocyte-specific mRNA probe ceruloplasmin (cp), and co-injection of TNF-α MO and TNF-α mRNA. An atonal homolog 7 (atoh7) mRNA probe was used to detect neurogenesis onset. The retinal neurodifferentiation was analyzed by immunohistochemistry using antibodies Zn12, Zpr1, and Zpr3 to label ganglion cells, con

SUBMITTER: Lei XD 

PROVIDER: S-EPMC4916138 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature