RNA-Seq Analysis of the Arabidopsis Transcriptome in Pluripotent Calli.

ABSTRACT: Plant cells have a remarkable ability to induce pluripotent cell masses and regenerate whole plant organs under the appropriate culture conditions. Although the in vitro regeneration system is widely applied to manipulate agronomic traits, an understanding of the molecular mechanisms underlying callus formation is starting to emerge. Here, we performed genome-wide transcriptome profiling of wild-type leaves and leaf explant-derived calli for comparison and identified 10,405 differentially expressed genes (> two-fold change). In addition to the well-defined signaling pathways involved in callus formation, we uncovered additional biological processes that may contribute to robust cellular dedifferentiation. Particular emphasis is placed on molecular components involved in leaf development, c

SUBMITTER: Lee K 

PROVIDER: S-EPMC4916400 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature