Androgen receptor regulates expression of the MUC1-C oncoprotein in human prostate cancer cells.

ABSTRACT:

Background

The MUC1 heterodimeric oncoprotein is aberrantly overexpressed in human prostate cancers with more aggressive pathologic and clinical features. However, the signals that regulate MUC1 expression in prostate cancer cells are not well understood.

Methods

MUC1 expression was studied in androgen-dependent and -independent prostate cancer cell lines by quantitative RT-PCR, immunoblotting and assessment of MUC1 promoter activation. Chromatin immunoprecipitation (ChIP) studies were performed to assess androgen receptor (AR) occupancy on the MUC1 promoter. Post-transcriptional regulation of MUC1 expression was assessed by miR-125b-mediated effects on activity of the MUC1 3' untranslated region (3'UTR).

Results

The present studies demonstrate that AR occupies a cons

SUBMITTER: Rajabi H 

PROVIDER: S-EPMC4916770 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature