Spatial relationship between chromosomal domains in diploid and autotetraploid Arabidopsis thaliana nuclei.

ABSTRACT: Polyploids constitute more than 80% of angiosperm plant species. Their DNA content is often further increased by endoreplication, which occurs as a part of cell differentiation. Here, we explore the relationship between 3D chromatin architecture, number of genome copies and their origin in the model plant, Arabidopsis thaliana. Spatial proximity between pericentromeric, interstitial and subtelomeric domains of chromosomes 1 and 4 was quantified over a range of distances. The results indicate that average nuclear volume as well as chromatin density increase with the genome copy number. Similar dependence is observed when association of homologous chromosomes (in 2C/ endopolyploid nuclei) and sister chromatid separation (in endopolyploid nuclei) is studied. Moreover, clusters of chromosomal

SUBMITTER: Sas-Nowosielska H 

PROVIDER: S-EPMC4916897 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature