Observing lysozyme's closing and opening motions by high-resolution single-molecule enzymology.

ABSTRACT: Single-molecule techniques can monitor the kinetics of transitions between enzyme open and closed conformations, but such methods usually lack the resolution to observe the underlying transition pathway or intermediate conformational dynamics. We have used a 1 MHz bandwidth carbon nanotube transistor to electronically monitor single molecules of the enzyme T4 lysozyme as it processes substrate. An experimental resolution of 2 μs allowed the direct recording of lysozyme's opening and closing transitions. Unexpectedly, both motions required 37 μs, on average. The distribution of transition durations was also independent of the enzyme's state: either catalytic or nonproductive. The observation of smooth, continuous transitions suggests a concerted mechanism for glycoside hydrolysis with lysoz

SUBMITTER: Akhterov MV 

PROVIDER: S-EPMC4916960 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature