3D tissue-engineered bone marrow as a novel model to study pathophysiology and drug resistance in multiple myeloma.

ABSTRACT:

Purpose

Multiple myeloma (MM) is the second most prevalent hematological malignancy and it remains incurable despite the introduction of several novel drugs. The discrepancy between preclinical and clinical outcomes can be attributed to the failure of classic two-dimensional (2D) culture models to accurately recapitulate the complex biology of MM and drug responses observed in patients.

Experimental design

We developed 3D tissue engineered bone marrow (3DTEBM) cultures derived from the BM supernatant of MM patients to incorporate different BM components including MM cells, stromal cells, and endothelial cells. Distribution and growth were analyzed by confocal imaging, and cell proliferation of cell lines and primary MM cells was tested by flow cytometry. Oxygen and drug grad

SUBMITTER: de la Puente P 

PROVIDER: S-EPMC4917006 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature