Novel Computational Protocols for Functionally Classifying and Characterising Serine Beta-Lactamases.

ABSTRACT: Beta-lactamases represent the main bacterial mechanism of resistance to beta-lactam antibiotics and are a significant challenge to modern medicine. We have developed an automated classification and analysis protocol that exploits structure- and sequence-based approaches and which allows us to propose a grouping of serine beta-lactamases that more consistently captures and rationalizes the existing three classification schemes: Classes, (A, C and D, which vary in their implementation of the mechanism of action); Types (that largely reflect evolutionary distance measured by sequence similarity); and Variant groups (which largely correspond with the Bush-Jacoby clinical groups). Our analysis platform exploits a suite of in-house and public tools to identify Functional Determinants (FDs), i.e.

SUBMITTER: Lee D 

PROVIDER: S-EPMC4917113 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature