Elucidating Proteoform Families from Proteoform Intact-Mass and Lysine-Count Measurements.

ABSTRACT: Proteomics is presently dominated by the "bottom-up" strategy, in which proteins are enzymatically digested into peptides for mass spectrometric identification. Although this approach is highly effective at identifying large numbers of proteins present in complex samples, the digestion into peptides renders it impossible to identify the proteoforms from which they were derived. We present here a powerful new strategy for the identification of proteoforms and the elucidation of proteoform families (groups of related proteoforms) from the experimental determination of the accurate proteoform mass and number of lysine residues contained. Accurate proteoform masses are determined by standard LC-MS analysis of undigested protein mixtures in an Orbitrap mass spectrometer, and the lysine count is

SUBMITTER: Shortreed MR 

PROVIDER: S-EPMC4917391 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature