Metabolomics meets functional assays: coupling LC-MS and microfluidic cell-based receptor-ligand analyses.

ABSTRACT:

Introduction

Metabolomics has become a valuable tool in many research areas. However, generating metabolomics-based biochemical profiles without any related bioactivity is only of indirect value in understanding a biological process. Therefore, metabolomics research could greatly benefit from tools that directly determine the bioactivity of the detected compounds.

Objective

We aimed to combine LC-MS metabolomics with a cell based receptor assay. This combination could increase the understanding of biological processes and may provide novel opportunities for functional metabolomics.

Methods

We developed a flow through biosensor with human cells expressing both the TRPV1, a calcium ion channel which responds to capsaicin, and the fluorescent intracellular calcium ion re

SUBMITTER: Henquet MGL 

PROVIDER: S-EPMC4917570 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature