Comprehensive mutation screening for 10 genes in Chinese patients suffering very early onset inflammatory bowel disease.

ABSTRACT:

Aim

To perform sequencing analysis in patients with very early-onset inflammatory bowel disease (VEO-IBD) to determine the genetic basis for VEO-IBD in Chinese pediatric patients.

Methods

A total of 13 Chinese pediatric patients with VEO-IBD were diagnosed from May 2012 and August 2014. The relevant clinical characteristics of these patients were analyzed. Then DNA in the peripheral blood from patients was extracted. Next generation sequencing (NGS) based on an Illumina-Miseq platform was used to analyze the exons in the coding regions of 10 candidate genes: IL-10, IL-10RA, IL-10RB, NOD2, FUT2, IL23R, GPR35, GPR65, TNFSF15, and ADAM30. The Sanger sequencing was used to verify the variations detected in NGS.

Results

Out of the 13 pediatric patients, ten were diagnosed

SUBMITTER: Xiao Y 

PROVIDER: S-EPMC4917618 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature