Dose-responsive gene expression in suberoylanilide hydroxamic acid-treated resting CD4+ T cells.

ABSTRACT:

Design

Persistent latently infected CD4 T cells represent a major obstacle to HIV eradication. Histone deacetylase inhibitors (HDACis) are a proposed activation therapy. However, off-target effects on gene expression in host immune cells are poorly understood. We hypothesized that HDACi-modulated genes would be best identified with a dose-response analysis.

Methods

Resting primary CD4 T cells were treated with 0.34, 1, 3, or 10 μmol/l of the HDACi, suberoylanilide hydroxamic acid (SAHA), for 24 h and subjected to microarray gene expression analysis. Genes with dose-correlated expression were filtered to identify a subset with consistent up or downregulation at each SAHA dose. Histone modifications were characterized in six SAHA dose-responsive genes by chromatin immunoprecip

SUBMITTER: Reardon B 

PROVIDER: S-EPMC4917679 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature