Structural and Kinetic Studies of Formate Dehydrogenase from Candida boidinii.

ABSTRACT: The structure of formate dehydrogenase from Candida boidinii (CbFDH) is of both academic and practical interests. First, this enzyme represents a unique model system for studies on the role of protein dynamics in catalysis, but so far these studies have been limited by the availability of structural information. Second, CbFDH and its mutants can be used in various industrial applications (e.g., CO2 fixation or nicotinamide recycling systems), and the lack of structural information has been a limiting factor in commercial development. Here, we report the crystallization and structural determination of both holo- and apo-CbFDH. The free-energy barrier for the catalyzed reaction was computed and indicates that this structure indeed represents a catalytically competent form of the enzyme. Comp

SUBMITTER: Guo Q 

PROVIDER: S-EPMC4917879 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature