Cryptosporidium as a testbed for single cell genome characterization of unicellular eukaryotes.

ABSTRACT:

Background

Infectious disease involving multiple genetically distinct populations of pathogens is frequently concurrent, but difficult to detect or describe with current routine methodology. Cryptosporidium sp. is a widespread gastrointestinal protozoan of global significance in both animals and humans. It cannot be easily maintained in culture and infections of multiple strains have been reported. To explore the potential use of single cell genomics methodology for revealing genome-level variation in clinical samples from Cryptosporidium-infected hosts, we sorted individual oocysts for subsequent genome amplification and full-genome sequencing.

Results

Cells were identified with fluorescent antibodies with an 80 % success rate for the entire single cell genomics workflow, d

SUBMITTER: Troell K 

PROVIDER: S-EPMC4917956 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature