Pre-Assembly of Near-Infrared Fluorescent Multivalent Molecular Probes for Biological Imaging.

ABSTRACT: A programmable pre-assembly method is described and shown to produce near-infrared fluorescent molecular probes with tunable multivalent binding properties. The modular assembly process threads one or two copies of a tetralactam macrocycle onto a fluorescent PEGylated squaraine scaffold containing a complementary number of docking stations. Appended to the macrocycle periphery are multiple copies of a ligand that is known to target a biomarker. The structure and high purity of each threaded complex was determined by independent spectrometric methods and also by gel electrophoresis. Especially helpful were diagnostic red-shift and energy transfer features in the absorption and fluorescence spectra. The threaded complexes were found to be effective multivalent molecular probes for fluorescen

SUBMITTER: Peck EM 

PROVIDER: S-EPMC4918808 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature