Sulfitolytic and keratinolytic potential of Chryseobacterium sp. RBT revealed hydrolysis of melanin containing feathers.

ABSTRACT: In black feathers, melanin is embedded in keratin matrix that makes feather more resistance to the microbial degradation. Chryseobacterium sp. RBT previously isolated from the poultry waste disposable site revealed strong sulfitolytic and keratinolytic activities. Maximum keratinase activity was observed at 48 h (89.12 U ml^-1) showed 83 % of native black feather degradation. The concentration of free sulfhydryl groups released during degradation was 0.648 × 10^-4 M (12 h), 2.144 × 10^-4 M (96 h), and however, declined on prolong incubation to 1.752 × 10^-4 M (120 h). Melanin was released in the degradation medium after microbial exploitation of black feather. After purification, melanin was dark brown colored powder insoluble in water, 5 M HCL, etha

SUBMITTER: Gurav RG 

PROVIDER: S-EPMC4919140 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature