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A click chemistry-based microRNA maturation assay optimized for high-throughput screening.

ABSTRACT: Catalytic enzyme-linked click-chemistry assays (cat-ELCCA) are an emerging class of biochemical assay. Herein we report on expanding the toolkit of cat-ELCCA to include the kinetically superior inverse-electron demand Diels-Alder (IEDDA) reaction. The result is a technology with improved sensitivity and reproducibility, enabling automated high-throughput screening.

SUBMITTER: Lorenz DA 

PROVIDER: S-EPMC4919152 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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A click chemistry-based microRNA maturation assay optimized for high-throughput screening.

Lorenz Daniel A DA   Garner Amanda L AL  

Chemical communications (Cambridge, England) 20160610 53

Catalytic enzyme-linked click-chemistry assays (cat-ELCCA) are an emerging class of biochemical assay. Herein we report on expanding the toolkit of cat-ELCCA to include the kinetically superior inverse-electron demand Diels-Alder (IEDDA) reaction. The result is a technology with improved sensitivity and reproducibility, enabling automated high-throughput screening. ...[more]

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