Antagonizing midkine accelerates fracture healing in mice by enhanced bone formation in the fracture callus.

ABSTRACT:

Background and purpose

Previous findings suggest that the growth and differentiation factor midkine (Mdk) is a negative regulator of osteoblast activity and bone formation, thereby raising the possibility that a specific Mdk antagonist might improve bone formation during fracture healing.

Experimental approach

In the present study, we investigated the effects of a monoclonal anti-Mdk antibody (Mdk-Ab) on bone healing using a standardized femur osteotomy model in mice. Additional in vitro experiments using chondroprogenitor and preosteoblastic cells were conducted to analyse the effects of recombinant Mdk and Mdk-Ab on differentiation markers and potential binding partners in these cells.

Key results

We demonstrated that treatment with Mdk-Ab accelerated bone healing i

SUBMITTER: Haffner-Luntzer M 

PROVIDER: S-EPMC4919577 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature