The Slow Mobility of the ParA Partitioning Protein Underlies Its Steady-State Patterning in Caulobacter.

ABSTRACT: In bacteria, ParABS systems mediate intracellular transport of various cargos, including chromosomal regions in Caulobacter crescentus. Transport of the ParB/parS partition complex requires the DNA-binding activity of ParA, which transiently tethers the partition complex during translocation. In C. crescentus, the directionality of the transport is set up by a gradient of ParA whose concentration gradually increases from one end of the cell (old pole) to the other (new pole). Importantly, this ParA gradient is already observed before DNA replication and segregation are initiated when the partition complex is anchored at the old pole. How such micron-scale ParA pattern is established and maintained before the initiation of chromosome segregation has not been experimentally established. Alth

SUBMITTER: Surovtsev IV 

PROVIDER: S-EPMC4919595 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature